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Determination of the limits of detection and quantification for the 1F5 indirect competitive ELISA.

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posted on 17.08.2021, 17:23 by Julieta S. Roldán, Alejandro Cassola, Daniela S. Castillo

The limit of detection (LOD) and the limit of quantification (LOQ) for the determination of denatured ZNS1 by icELISA with 1F5 mAb were obtained measuring the concentration of 6 different normal human sera (blank samples) and calculated by the following formulas: LOD = mean blank samples±3*SD blank samples, and LOQ = mean blank samples±10*SD blank samples. The dotted line represents the mean of the blank samples analyzed.