Determination of the limit of detection (LOD) of the developed NALFA, for RdRp gene.
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posted on 2024-05-10, 17:27 authored by João M. Vindeirinho, Ricardo Oliveira, Eva Pinho, Raquel Guiomar, Nuno F. Azevedo, Carina AlmeidaA range of initial RNA concentrations expressed as the total number of copies between 0 (NTC) and 2000 were used for RT-PCR amplification; being then used to assess the LOD of the developed NALFA.
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twenty clinical sampleslateral flow stripgold standard rtclinical specimens tested25 rna copiesdna probes immobilizedoriginating equal resultsviruses like sars2 gene tailedstrand dnarelated virusestailed primersthus overcomingtailed ampliconssuccessfully implementedspecific sarssimultaneously comparedrelatively smallreaction mixqpcr protocolpromising solutiongreat dependencyeffective readingsamplification protocolsamplification efficiencyaforementioned problemsaccurate route2 diagnosis100 %.
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