Detection of IgM/IgG antibodies from WNV, ZIKV and DENV 1–4 infected individuals.
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A and B) Detection of IgM (A) IgG (B) antibodies by rNS1-based ELISA. Each group of RT-PCR confirmed patients were screened for the presence of IgM/IgG antibodies by rNS1-based ELISA tests using specific purified rNS1 antigens. Optimal cut-off values of P/N ratios were calculated based on ROC curve analysis. Each cut-off was selected based on the P/N ratio value, which gave 100% sensitivity and specificity. * each ELISA result includes the average of two biological replicates.