Delayed development in rpS13/+ animals is due to impaired ecdysone production.

(A) qRT-PCR measurements of phantom and spookier mRNA levels in w¹¹¹⁸ and rpS13/+ larvae. n = 4 independent samples per genotype. Data are presented as +/- SEM. *p < 0.05, two-way ANOVA and post-hoc Student’s t-test. (B) Time to pupation of w¹¹¹⁸ and rpS13/+ larvae grown in control food or food supplemented with ecdysone (20HE). Data are presented as +/- SEM. *p < 0.05, Mann-Whitney U test. n = 142 (+/+), 92 (+/+ with 20HE), 148 (rpS13/+), 116 (rpS13/+ with 20HE). (C) Time to pupation of +/+ and rpS13/+ larvae with or without UAS-rheb expression in the prothoracic gland using P0206-Gal4. Data are presented as +/- SEM. ns = not significant, *p < 0.05, Mann-Whitney U test. n = 152 (+/+), 157 (UAS-rheb/+), 151 (rpS13/+), 144 (UAS-rheb/rpS13).