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Defining the detection limit of the PCR system

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posted on 2011-12-30, 14:32 authored by Angelika Lehner, Taurai Tasara, Roger Stephan

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Taken from "16S rRNA gene based analysis of strains from different sources and development of a PCR assay for identification"

BMC Microbiology 2004;4():43-43.

Published online 25 Nov 2004

PMCID:PMC538263.

Copyright © 2004 Lehner et al; licensee BioMed Central Ltd.

Decreasing amounts of purified strain ATCC 51329 genomic DNA target (100 ng – 1 pg) were amplified by PCR. Lane 1, DNA 100 bp marker; In lanes 2 to 5, 1 ng, 100 pg, 10 pg, 1 pg of DNA was used per reaction.

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