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DNA damage analysis.

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posted on 10.01.2020, 18:45 by Emilio Rojas, Monica Martinez-Pacheco, Maria Alexandra Rodriguez-Sastre, Paulina Ramos-Espinosa, Mahara Valverde

(A) DNA DSBs in irradiated Balb/c 3T3 cells previously transfected with pre-miR-222 molecules at 5 min (3 Gy), 1 h post-exposure and evaluated with the neutral comet assay protocol. Results are presented in terms of Olive Tail Moment (OTM); dose of gamma radiation = 3 Gy, N = 2 slides/condition, 50 comets/slide, mean ± SE, two-tailed unpaired t-test, *p<0.05. (B) Distribution of DNA damage from pre-miR-222 cells treated with gamma-radiation (3Gy) and 1 h post-exposure, and analyzed by neutral comet assay.

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