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DAPI staining reveals larger nuclei in TERΔ diploids.

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posted on 2025-04-02, 17:28 authored by Jessica K. Day, Brett J. Palmero, Amanda L. Allred, Junya Li, Fatima B. Hooda, Graeme Witte, Alexandra M. Dejneka, Anna M. Sandler, Karen E. Kirk

A. Representative DAPI-stained conidia from a TERΔ diploid transformant colony and a parent haploid SO451 colony. The DAPI (left) and merge (right) images are shown for each conidium. B and C. Bar graphs depicting the (B) mean intensity and (C) mean volume (nucleus size) for TERΔ (shades of blue) and the haploid SO451 strain (red/orange) with standard error bars. Results show that TERΔ transformants have nuclei nearly double the size of the SO451 DAPI-stained nuclei. The DAPI stain intensity for the TERΔ background is approximately 1.5 times that of the SO451. ( * =  p <  0.05, ** =  p <  0.005).

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