Cytotoxicity assessment of NMDI-1 treatment.
(A, B) Flow cytometry analysis of virus-negative Burkitt lymphoma BJAB (A) or AGS (B) cells treated with 5–100 μM NMDI-1 or DMSO (“0 μM” NMDI-1) for 48 hours and stained with 7-AAD and FITC-Annexin V. Data are presented as mean percentage of live (orange, Annexin V-/7-AAD-), apoptotic (blue, Annexin V+/7-AAD-), and necrotic (red, Annexin V+/7-AAD+) cells for 3 biological replicates. Moreover, 1 μM staurosporine treatment served as positive control for apoptosis/necrosis induction. The underlying numerical data can be found in S1 Data. NMD, nonsense-mediated decay.