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Cytokine content in the membranes of activated Th1 and Th2 cell clones

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posted on 30.12.2011, 16:13 by Carlo Chizzolini, Yann Parel, Agneta Scheja, Jean-Michel Dayer

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Taken from "Polarized subsets of human T-helper cells induce distinct patterns of chemokine production by normal and systemic sclerosis dermal fibroblasts"

Arthritis Research & Therapy 2005;8(1):R10-R10.

Published online 30 Nov 2005

PMCID:PMC1526552.

Cytokine levels were determined by enzyme-linked immunosorbent assay in Th cell membranes. Th cells were activated by CD3 cross-linking for six hours before membrane preparation. The bars represent the mean ± standard deviation of six Th1 and six Th2 cell clones, and indicate the cytokine levels in the cell membranes from 2 × 10cells. When a given cytokine was not detectable, it was assigned the value 0 for mean determination. *< 0.05. IFN, interferon; IL, interleukin; ND, not detectable; Th, T-helper; TNF, tumour necrosis factor.

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