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Cumulative distribution functions (CDF) of residence times.

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posted on 2017-02-06, 19:05 authored by Katrin Schenk, Ana B. Hervás, Thomas C. Rösch, Marc Eisemann, Bernhard A. Schmitt, Stephan Dahlke, Luise Kleine-Borgmann, Seán M. Murray, Peter L. Graumann

The plots show the cumulative probability that a molecule remains in a circle of radius 230 nm for the indicated time. A) Comparison of YFP-DnaA expressed from the ectopic amyE locus under the control of a xylose inducible promoter (ME15) and YFP-YabA expressed from the ectopic amyE locus under the control of a xylose inducible promoter in a strain deleted for YabA. B) Comparison of ME15 and YFP-Spo0J expressed from the ectopic amyE locus under the control of a xylose inducible promoter C) Comparison of ME15 and YFP-DnaA expressed from the ectopic amyE locus under the control of a xylose inducible promoter in a strain lacking Soj-Spo0J. D) YFP-DnaA (ME15) compared with YFP-DnaAE183Q expressed from the ectopic amyE locus under the control of a xylose inducible promoter. E) YFP-DnaA (ME15) compared with E. coli DnaA-YFPsw expressed from the native locus. In all comparisons, the null hypothesis that the data of the curves come from the same distributions is rejected at the 5% significance level (two-sample Kolmogorov-Smirnov (KS) -test).

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