Cryo-EM structures of F4-Fab-HAV complex, F6-Fab-HAV complex, F7-Fab-HAV complex, and F9-Fab-HAV complex.
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posted on 2019-04-30, 17:25 authored by Lei Cao, Pi Liu, Pan Yang, Qiang Gao, Hong Li, Yao Sun, Ling Zhu, Jianping Lin, Dan Su, Zihe Rao, Xiangxi Wang(A) The maps of F4, F6, F7, and F9 Fab in complex with full particles are colored based on the radial distance (Å) of the capsid and Fab from the particle center. Only the front half of each type of particle is shown. Triangles indicate an icosahedral asymmetric unit. (B) The typical NAb’s Fab (F7) binds to the viral surface along the pentamer interface between the 2-fold and 3-fold axes. (C) The quality of Fab-HAV complex density map (gray) is illustrated by fit of backbone and side chains for 3 separate structures of F7-Fab-HAV complex. cryo-EM, cryo-electron microscopy; Fab, fragment of antigen binding; HAV, hepatitis A virus; Nab, neutralizing monoclonal antibody.
