pone.0215104.g002.tif (2.08 MB)

Correlation between cumulative EPSC occurrence and the number of cells died due to ischemic insult in hippocampal CA1 area.

Download (2.08 MB)
posted on 12.04.2019, 17:25 by Shohei Yokoyama, Ichiro Nakagawa, Yoichi Ogawa, Yudai Morisaki, Yasushi Motoyama, Young Su Park, Yasuhiko Saito, Hiroyuki Nakase

A. Microscopic images of CA1 area showing nuclei of dead cells. Magenta-colored cells stained with both propidium iodide and SYTOX-blue were considered to have died before electrophysiological recording. Blue-colored cells stained with only SYTOX-blue were assumed to have died due to ischemia-reperfusion injury. Scale bars = 50 μm. B. Two-dimensional scatter plot of cumulative EPSC occurrence and dead cell number per 1 mm of CA1 area. A positive correlation between cumulative EPSC occurrence and surviving cell number was statistically significant (Pearson’s correlation coefficient r = 0.70, n = 11, Student’s t = 2.96, p = 0.016). The line represents an estimated linear regression.