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Coreceptor tropism of early SHIV stocks.

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posted on 2016-02-05, 19:49 authored by Lawrence J. Tartaglia, Hui-Wen Chang, Benjamin C. Lee, Peter Abbink, David Ng’ang’a, Michael Boyd, Christy L. Lavine, So-Yon Lim, Srisowmya Sanisetty, James B. Whitney, Michael S. Seaman, Morgane Rolland, Sodsai Tovanabutra, Jintanat Ananworanich, Merlin L. Robb, Jerome H. Kim, Nelson L. Michael, Dan H. Barouch

(A-B) TZM-bl cells were incubated for 1 h with different concentrations of the CCR5 inhibitor TAK-779 or the CXCR4 inhibitor AMD-3100 and subsequently were infected with 100 TCID50 of the indicated SHIV stocks. The luciferase activity was quantified after 48 h. (C) GHOST cell lines expressing CXCR4 (X4) and/or CCR5 (R5) coreceptors were used, and inoculated with 100-TCID50 SHIV stocks. Cell culture supernatant was collected for SIV p27 determination after 4 days of infection. All assays were done in triplicate. The means with standard deviation are shown.