pgen.1006490.g005.tif (4.61 MB)

Coordinated expression of central carbon metabolic pathways.

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posted on 2016-12-14, 12:23 authored by Sarah R. Smith, Jeroen T. F. Gillard, Adam B. Kustka, John P. McCrow, Jonathan H. Badger, Hong Zheng, Ashley M. New, Chris L. Dupont, Toshihiro Obata, Alisdair R. Fernie, Andrew E. Allen

(A) Biological replicate averaged transcript abundance (for each time point) shown for genes from the Calvin Benson cycle, mitochondrial glycolysis pathway, cytosolic glycolysis pathway, and TCA cycle for each Fe condition (error bars omitted for clarity). Series labeled with gene names (see S4 Dataset for abbreviations) and Phatr3 PID. (B) Schematic representation of reactions catalyzed by GAPDH and PGK across the cytosol, mitochondria, and chloroplast (shown with 4 membranes). NADPH and ATP produced from light harvesting depicted in green, and NAD(P)H and ATP regenerated in the cytosol or mitochondria depicted in magenta. Yellow/blue highlighted arrows indicate day/night upregulation respectively. Solid arrows indicate likely transport processes, dashed arrow indicates hypothesized carbon skeleton transport. GAP: glyceraldehyde 3-phosphate, 3PG: 3-phosphoglycerate, FAB: fatty acid biosynthesis. (C) Transcript abundance for chloroplast, mitochondrial, and cytosolic targeted isoforms of glyceraldehyde phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK) are plotted (TPI-GAPDH: triosephosphate isomerase-GAPDH fusion protein).