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Conformational stability of BSA-AuNC complex.

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posted on 2019-06-27, 17:48 authored by Michał Kluz, Hanna Nieznańska, Robert Dec, Igor Dzięcielewski, Bartosz Niżyński, Grzegorz Ścibisz, Wojciech Puławski, Grzegorz Staszczak, Ewelina Klein, Julita Smalc-Koziorowska, Wojciech Dzwolak

Titration of BSA-AuNC, native BSA, and BSA-Alk with GdnHCl at pH 7 and 25 oC monitored with far-UV CD. In the bottom right panel, quantitative plots correspond to GdnHCl-dependences of CD signals at 225 nm. Plasticity of secondary structures decreases in the order: BSA-AuNC > BSA-Alk > native BSA. The corresponding low GdnHCl concentration range is marked with the shadowed area.

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