Comparisons of raw intensity differences from CRR reference instrument, generated from beads and sample harmonization methods at A) Health Canada and B) Flow Core.

MFI values from each harmonized sample (unstained and AF488only—0 Gy, as well as BAX and p53–0 and 3 Gy) were subtracted from values of corresponding replicate at CRR. n = 4; ns (not significant) reflects the significance of Wilcoxon matched-pairs signed rank test.

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