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Comparisons of IL-10 and transforming growth factor (TGF)-β production by mononuclear lymphocytes from lymphoid organs of tolerized and nontolerized mice

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posted on 31.12.2011, 04:37 by So-Youn Min, Sue-Yun Hwang, Kyung-Su Park, Jae-sun Lee, Kang-Eun Lee, Kyung-Wun Kim, Young-Ok Jung, Hyunk-Jae Koh, Ju-Ho Do, Haerim Kim, Ho-Youn Kim

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Taken from "Induction of IL-10-producing CD4CD25T cells in animal model of collagen-induced arthritis by oral administration of type II collagen"

Arthritis Research & Therapy 2004;6(3):R213-R219.

Published online 11 Mar 2004


Copyright © 2004 Min et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Organs were extracted from normal DBA/1, and tolerized and nontolerized collagen-induced arthritis (CIA) mice at 5 weeks after primary immunization with type II collagen (CII) for induction of CIA. Mononuclear cells were isolated from the Peyer's patch and the spleen, and production of IL-10 and TGF-β was assessed by sandwich ELISA analyses of culture supernatants. The data represent average values from three independent measurements.