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Comparison of the yields of purified scFv -/+ CyDisCo.

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posted on 18.12.2017, 18:31 by Anna Gąciarz, Lloyd W. Ruddock

Yields in mg/L/OD600 of scFv expressed with CyDisCo (black bars) or without (white bars). Average OD600 of the cultures was 22 ± 3 for those containing CyDisCo and 27 ± 3 for cultures without CyDisCo. Error bars show standard deviation, n = 3–4. scFv frameworks which have all four cysteines mutated to alanine are indicated as CA.

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