Comparison of the yields of purified scFv -/+ CyDisCo.
Yields in mg/L/OD600 of scFv expressed with CyDisCo (black bars) or without (white bars). Average OD600 of the cultures was 22 ± 3 for those containing CyDisCo and 27 ± 3 for cultures without CyDisCo. Error bars show standard deviation, n = 3–4. scFv frameworks which have all four cysteines mutated to alanine are indicated as CA.