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Comparison of steady-state levels of representative mRNAs in wild-type (MG1693) and PAP I induced (SK9124) strains employing northern blot analysis

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posted on 31.12.2011, 02:55 by Bijoy K. Mohanty, Sidney R. Kushner

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Taken from "The majority of mRNAs undergo post-transcriptional modification in exponentially growing cells"

Nucleic Acids Research 2006;34(19):5695-5704.

Published online 12 Oct 2006

PMCID:PMC1636475.

© 2006 The Author(s)

Cell cultures were grown to 50 Klett units (∼1 × 10cells/ml) above background (0 min). Subsequently IPTG (350 μM) was added and the cultures were grown for additional times. Total RNA was isolated at times (min after IPTG addition) indicated on the top of the blot. Five microgram of total RNA was loaded in each lane and separated in 6% polyacrylamide/7 M urea gels. The transcripts were probed as described in Materials and Methods. The relative quantity of each full-length transcript was determined using a Molecular Dynamics PhosphorImager. The wild-type level of each mRNA at 0 min was set at 1 and the corresponding fold-changes are noted at the bottom of each lane.

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