Comparison of Kpe BMC-H sequences.

A. Sequence alignments were prepared with the RCSB pairwise alignment tool (TM-align method), taking as input the structures from the BMC-H monomers generated by AF2. Secondary structure elements from PduA are indicated as reference. EutS and PduU present secondary structure permutations. They were aligned to PduA using the JCE-CP (flexible) method and manually verified. The permutation is highlighted by the black rectangle, indicating the number of the first shown residue. PduU and EutS residues that build the N-terminal β-barrel were excluded. Residues fully or partly embedded at the interface between monomers are indicated by black or grey arrows, respectively. Critical lysine (K26 in PduA) and pore residues that also participate to the interface are indicated by blue and green arrows, respectively. The presentation was generated online with ESPript 3.0. B. Percentage of sequence identity between Kpe BMC-H. Values based on either residues belonging to the common BMC-H core domain (left) or only those falling at the interface between monomers (right). Coloured values are to highlight cases exhibiting more than 10% discrepancy of identity when comparing the two sets of residues.

(TIF)