Comparison of CZA97.012 SOSIP.664 trimer produced in different cell lines and differentially affinity-purified.
Trimer produced in either a stable CHO cell line or by transient transfection of HEK-293F cells was 2G12-, PGT145-, or PGT151-affinity- and then SEC-purified. Site-specific glycan content was determined by LC-MS on intact glycopeptides as in Fig 8B, but here the contents of oligomannose-type glycans were summed up, i.e., only Man9GlcNAc2, Man8GlcNAc2, Man7GlcNAc2, Man6GlcNAc2, and Man5GlcNAc2 together, and expressed as percentages of total glycan per site. The bar charts thus represent the relative accumulated oligomannose-type glycan content at each PNGS in CZA97.012 SOSIP.664. Light green represents CHO-derived and dark green HEK-293F-derived trimer (see legend). Sites for which glycopeptides of sufficient quality could not be obtained are left non-determined and are marked with asterisks.