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Comparing C. muridarum with or without plasmid for their ability to spread to the GI tracts of CBA/1J mice following an intravaginal inoculation.

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posted on 25.05.2017 by Lili Shao, Jose Melero, Nu Zhang, Bernard Arulanandam, Joel Baseman, Quanzhong Liu, Guangming Zhong

The experiments were carried out and the results were presented as described in Fig 1 legend above. The plasmid-competent C. muridarum organisms were inoculated into 5 while the plasmid-deficient to 10 mice. The data were collected from 2 independent experiments. Plasmid-deficient C. muridarum displayed significantly longer shedding from the mouse genital tracts (panel a, p<0.05, days to clearance, Wilcoxon rank sum) but the overall shedding courses were similar between plasmid-deficient and -competent C. muridarum (panels a & b, p>0.05, area under the curve, Wilcoxon rank sum). However, plasmid-deficient C. muridarum developed significantly delayed/reduced shedding courses in the GI tracts in terms of both the level of shedding (panel c, **p<0.01, area under the curve, Wilcoxon rank sum) and number of mice remaining positive for shedding (panel d, *p<0.05, areas under the curve, Wilcoxon rank sum).