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Comparative analysis of ABA sensitivity of wild-type Arabidopsis and DNA double-strand break (DSB)-repair related mutants in presence of exogenously applied abscisic acid during seed germination.

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posted on 2017-01-03, 18:56 authored by Sujit Roy, Kali Pada Das

Arabidopsis seeds from the wild type (Wt Col) and DSB-related mutants were germinated on MS-agar medium containing different concentrations of ABA. Plates were kept in the dark at 4°C for 3–5 days and then transferred to light chambers maintained at 22°C with 16-h-light/8-h-dark periods, respectively. One-week-old plants were tested for their sensitivity. (A) Satatistical analyses of germination rates in wild-type and DSB-related mutant plants grown in the absence or presence of increasing concentrations of ABA. (B) Similar experimental conditions were used as in (A) for analyzing the germination rates of wild-type and other DSB-related mutant lines. The bars represent mean values of from three independent observations. *P < 0.05, **P < 0.01 relative to respective controls (n = 3). In each case approximately 75 seeds were counted.