Cold digestion with dispase II does not remove MHC-I, MHC-II or CD24 from the surface of CD45-CD31-EpCAM+ cells.
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posted on 2024-01-25, 18:29 authored by Piotr Pawel Janas, Caroline Chauché, Patrick Shearer, Georgia Perona-Wright, Henry J. McSorley, Jürgen SchwarzeFollowing debris exclusion (FSC/SSC), singlets gating (FSC-H/FSC-A), live cells gating (LIVE/DEAD fixable near-IR/FSC-H), CD45 and CD31 exclusion (CD45/CD31) and EpCAM+ gating (EpCAM/FSC-H) expression of MHC-I, MHC-II and CD24 was evaluated. Gating was based on fluorescence minus one (FMO) controls.
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sup >-sup >+pulmonary immune responsesmaintaining lung homeostasisclassically used shortactivated cell sortingdispase ii digestionstudying lung aecsfold higher numberefficient aecs isolationcold dispase digestioncold digestion allowsdiv >< pairway epithelial cellsviable aecs compareddigestion protocolsgreater numbervitro utilizing longprotocol yieldsmurine lungsmacs ),larger numbersincreased comparedimmunofluorescent microscopygreater numbersflow cytometryestablished protocolsestablished methodepithelium regenerationconsiderable impactaec yield
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