Co-culture electrophysiological reproducibility between two 24-well plates.
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posted on 2024-06-25, 17:33 authored by Maddie R. Lemieux, Bernhard Freigassner, Jessica L. Hanson, Zahra Thathey, Mark R. Opp, Charles A. Hoeffer, Christopher D. LinkCharacterized by (A) Mean firing rate (Hz) (B) Number of active electrodes (C) Network burst frequency (Hz) (D) Average network burst duration (s) (E) Network interburst interval coefficient of variation (F) Synchrony index. Statistical analysis was performed with an independent t-test. Error bar: SEM. N = 24 independent wells per plate. p-value < 0.05 *, p-value < 0.01 **, p-value < 0.001 ***.
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∼ 13 hzusing multielectrode arraysusing human serumtake several monthsstudying network propertiesprimary human astrocytesmultielectrode array characterizationmean firing rateinducible neurogenin 2excellent first passbrain barrier breakdownstudy cellular processestime without disruptionprevious reports showfully human codiv >< pused wtc11 hipscsprevious reportscellular culturesalso usedvitro transgene differentiatedthroughput methodone methodneurons offerneurons coextended periodselectrophysiological activity
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