ppat.1007713.g002.tif (2.66 MB)

ClfB contributes to abscess structure and size during S. aureus SSTI.

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posted on 22.04.2019 by Keenan A. Lacey, Michelle E. Mulcahy, Aisling M. Towell, Joan A. Geoghegan, Rachel M. McLoughlin

BALB/c mice were infected subcutaneously with 2x107 CFU S. aureus LAC::lux or LAC::lux ΔclfB and abscess tissue was excised at 12, 48 and 96 hours. Tissue was fixed, embedded in paraffin wax and sectioned before haematoxylin and eosin staining was performed. Representative sections from each group are shown (A). White arrows indicate the lack of defined abscess wall structure. Tissue sections were scored (double blind) for histology score (B). Abscess area was computed using ImageJ software and the areas were scored accordingly (C). Results expressed as mean score ± SEM. n = 6 per group. Data pooled from 2 independent experiments. Two-way ANOVA with Bonferroni post-test used to analyze differences between groups (B, C). * P < 0.05, ** P < 0.01.