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Chitin-resin pull down with ChiA mutants.

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posted on 04.05.2020, 17:39 by Saima Rehman, Lubov S. Grigoryeva, Katherine H. Richardson, Paula Corsini, Richard C. White, Rosie Shaw, Theo J. Portlock, Benjamin Dorgan, Zeinab S. Zanjani, Arianna Fornili, Nicholas P. Cianciotto, James A. Garnett

SDS-PAGE gels loaded with ChiA-CTD mutants or BSA control either before incubation with chitin beads (L) or after elution from the beads (B). Eluted samples undergo an upward shift compared to the input sample due to differences in buffer conditions. Data is representative of three independent repeat experiments.