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Chitin-induced polymerization of Mg1LysM and RiSLM, but not of Ecp6.

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posted on 23.06.2020, 17:49 by Andrea Sánchez-Vallet, Hui Tian, Luis Rodriguez-Moreno, Dirk-Jan Valkenburg, Raspudin Saleem-Batcha, Stephan Wawra, Anja Kombrink, Leonie Verhage, Ronnie de Jonge, H. Peter van Esse, Alga Zuccaro, Daniel Croll, Jeroen R. Mesters, Bart P. H. J. Thomma

Effector proteins were incubated with chitohexaose (chitin) or water as control. After overnight incubation, methylene blue was added and protein solutions were centrifuged, resulting in protein pellets as a consequence of polymerization for Mg1LysM and RiSLM, but not for Ecp6. NP is the ‘no protein’ sample with chitin in the absence of LysM effector protein.