Characterizing D1R signaling to cAMP in dMSN KCTD5 knockout.

(A) Average response traces of evoked dopamine (20 Hz, 20 pulses) to a paired-pulse protocol with 30 min between recordings in the presence (24 neurons/6 animals) or absence (28 neurons/6 animals) of SCH23390 (10 μM) in Drd1a^Cre:CAMPER^+/+ slices. (B) Maximum cAMP response to 20 Hz paired-pulse stimulation in dMSN. Buffer: Nonparametric t test, Mann–Whitney U = 312.5, p = 0.1956. SCH23390: Nonparametric t test, Mann–Whitney U = 0, p < 0.0001. (C) Average response traces to vary stimulation frequencies (20 pulses) in dMSN WT (5 animals, ≥24 neurons/frequency) and dMSN KO (5 animals, ≥27 neurons/frequency). All data presented as mean ± SEM. The numerical data presented in this figure can be found in S2 Data.

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