Characterization of stable incorporation of pCRISPR-EASY into the genome in blasticidin resistant monoclonal cell lines.
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posted on 2024-03-14, 17:40 authored by Mohammad J. Hossain, Tamara J. O’ConnorPCR amplification of a region of pCRISPR-EASY from isolated genomic DNA of was used to assay for chromosomal integration of pCRISPR-EASY in 14 individual blasticidin resistant monoclonal RAW264.7 cell lines generated using Pmp70 sgRNA-1 (S1 Table). pCRISPR-EASY was used as a positive control.
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