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Characterization of Ins1-/-:Ins2+/+ and Ins1-/-:Ins2+/- Islets.

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posted on 2016-04-07, 05:16 authored by Nicole M. Templeman, Arya E. Mehran, James D. Johnson

Separate groups of male mice (discrete from cohorts A and B) were used for islet analyses. (a) In vivo 4-h fasted insulin is shown from 25 week-old mice prior to collection of islets to assess (b) Ins2 mRNA, corrected against ß-actin and normalized to CD-fed Ins1-/-:Ins2+/+ mice, (c) islet insulin content, and (d) insulin secretion by 150 islets perifused with 3 mM glucose (basal), 15 mM glucose (Glu), and 30 mM KCl, with area under the curve (y-axis units of ng/mL•min) depicted for phases I/II of glucose and KCl stimulation, minus basal secretion. (e) in vivo 4-h fasted insulin is shown from 52 week-old mice prior to collection of islets at 70 weeks to assess (f) Ins2 mRNA, corrected against ß-actin and normalized to HFD-fed Ins1-/-:Ins2+/+ mice, (g) islet insulin content, and (h) insulin secretion by 150 islets perifused with 3 mM glucose (basal), 15 mM glucose (Glu), and 30 mM KCl, with area under the curve (y-axis units of ng/mL•min) depicted for phases I/II of glucose and KCl stimulation, minus basal secretion. n = 3–7. Data are means ± SEM, with scatter points to indicate individual values. Dark blue and red represent CD- and HFD-fed Ins1-/-:Ins2+/+ male mice, respectively; pale blue and orange represent CD- and HFD-fed Ins1-/-:Ins2+/- male mice, respectively. p ≤ 0.05 denoted by * for CD vs HFD, # for Ins1-/-:Ins2+/+ vs Ins1-/-:Ins2+/-. (#) indicates p = 0.056 for Ins1-/-:Ins2+/+ vs Ins1-/-:Ins2+/-.

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