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Characterization of chimeric HPV16L1-17RG1 and 5L1-17RG1 VLP by ELISA.

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posted on 05.01.2017, 18:57 by Bettina Huber, Christina Schellenbacher, Saeed Shafti-Keramat, Christoph Jindra, Neil Christensen, Reinhard Kirnbauer

(A) HPV16L1-17RG1 VLP were compared to wt HPV16 L1 VLP under native conditions using neutralizing mAb H16.V5 and H16.E70, and non-neutralizing mAb Camvir-1. (B) Serial dilutions of antisera raised against HPV16L1-17RG1 or HPV5L1-17RG1 were analyzed in triplicates by HPV17RG1 peptide ELISA. Serum titers are reported for OD values greater than the mean OD of pre-immune sera plus 3 standard deviations (SD).