pone.0217897.g002.tif (6.63 MB)

Characterization of an Nsp1FG surface by AFM.

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posted on 2019-06-06, 17:48 authored by Ryo Hayama, Mirco Sorci, John J. Keating IV, Lee M. Hecht, Joel L. Plawsky, Georges Belfort, Brian T. Chait, Michael P. Rout

(A) Schematic diagram of Nsp1FG approach-pulling experiment and worm-like chain (WLC) model parameters. (B) Representative retraction curves during single Nsp1FG stretching (left), and superimposition of the normalized curves (right). Each curve represents a single stretching event. (C) WLC model estimates of persistence length (Lp) and contour length (Lc) scatter-plotted against the adhesion force. Their frequency distributions are plotted in respective histograms. (D) Approach curves for layer thickness measurements: Control (PEG-PEG, left), single Nsp1FG layer (PEG-Nsp1, middle), and double Nsp1FG layer (Nsp1-Nsp1, right).