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Cell viability assay using cancer cell lines.

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posted on 2020-01-10, 18:41 authored by Ryotaro Ohkuma, Erica Yada, Shumpei Ishikawa, Daisuke Komura, Hidenobu Ishizaki, Koji Tamada, Yutaro Kubota, Kazuyuki Hamada, Hiroo Ishida, Yuya Hirasawa, Hirotsugu Ariizumi, Etsuko Satoh, Midori Shida, Makoto Watanabe, Rie Onoue, Kiyohiro Ando, Junji Tsurutani, Kiyoshi Yoshimura, Takehiko Yokobori, Tetsuro Sasada, Takeshi Aoki, Masahiko Murakami, Tomoko Norose, Nobuyuki Ohike, Masafumi Takimoto, Masahiko Izumizaki, Shinichi Kobayashi, Takuya Tsunoda, Satoshi Wada

(a) Schematic representation of the procedure. Expression of the control vector and OLFM4 was induced in indicated cell lines. After 24 h (day 1), GEM was added at various concentrations. Cell viability assays were performed 48 h after GEM administration (day 3). (b and c) Rate of change of each measured OD value of the control vector and OLFM4-expressing HeLa cells (b) and MIA Paca2 cells (c) is shown. (d) Rate of change of each measured OD value of siRNA negative control and specific siRNA targeting OLFM4 induced in SUIT-2 cells is shown.

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