Cell proliferation of PDX-derived CRC cell lines treatment combinations.
(A) Western blot analysis of PDX-derived CRC cell lines treated with 10μM FND-4b, 5μM PI-103, and 100nM SN-38, alone and in combination versus untreated control (media alone) for 24h duration. β-actin was used as a loading control. The images are representative of three independent experiments. In all CRC cell lines mono- or dual-treatment with FND-4b resulted in decreased cyclin D1 expression. (B) SRB Cytotoxicity Assays of PDX-derived CRC cell lines treated with 10μM FND-4b, 5μM PI-103, and 100nM SN-38, alone and in combination for 144h duration. Graphic representations are the mean ± SD plotted as a percentage relative to untreated control; each measurement was performed with 5 replicates. *p<0.0001 vs. control, #p<0.01 vs. FND-4b alone, and +p<0.05 vs. SN-38 alone.