pone.0197184.g001.tif (1.51 MB)

Calibration curve of qPCR singleplex and duplex assays with tenfold serially diluted 16S and RNR plasmid DNA (1.48E+05 to 1.48E+00 copies/μl and 1.47E+05 to 1.47E+00 copies/μl, respectively) using 16S (broken line) and RNR (unbroken line) primers at 100:200 nm primer:probe ratio.

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posted on 17.05.2018, 08:18 by Vijayanandraj Selvaraj, Yogita Maheshwari, Subhas Hajeri, Jianchi Chen, Thomas Greg McCollum, Raymond Yokomi

(a) The singleplex qPCR assay efficiency for 16S and RNR Plasmid DNA standard curve is 98.34%, 95.21%, respectively, and (b) for duplex qPCR assay is 99.37%, 98.97%, respectively.

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