CaMKII blockers (KN93 left; tatCN21 right) act through a whole-cell twofold division of the AMPA single channel conductance in a subset of cells from trained rats.

A, E For each cell, the effect of CaMKII blocker on the average and standard deviation of the AMPA mediated event amplitudes is shown. The effect of the CaMKII blocker exhibits a clear division into two clusters (KN93: trained, n = 16; pseudo, n = 6; naïve, n = 14. tatCN21: trained, n = 7; pseudo, n = 5; naïve, n = 7). B, F Similarly, the effect of CaMKII blocker on the AMPA mediated average event amplitude and AMPAR single channel current exhibits two clusters (KN93: trained, n = 10; pseudo, n = 6; naïve, n = 10. tatCN21: trained, n = 6; pseudo, n = 3; naïve, n = 4). C, G A distribution curve of the AMPA mediated event amplitude averaged for all cells in the affected cluster recorded before and after the CaMKII blocker (KN93: n = 5; tatCN21: n = 4). The black curve computes the reverse of the blocker effect under the multiplication model after calculating for each cell the fraction of events that were affected by the blocker (see Fig 1C and 1G). D, H the blocker effect could be reversed for each cell separately. Left: an example of one cell; right: the P value was calculated for each cell and the average of these P values was calculated from all cells in the affected cluster.