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COS-1 cells transfected with wild type or Δ2–111 ORF2 expression plasmids were lysed 48 hours post-transfection, the lysates cross-linked with glutaraldehyde and separated on a sucrose gradient as described in materials and methods

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posted on 2011-12-30, 22:12 authored by Li Xiaofang, Mohammad Zafrullah, Faizan Ahmad, Shahid Jameel

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Taken from "A C-terminal hydrophobic region is required for homo-oligomerization of the hepatitis E virus capsid (ORF2) protein"

Journal of Biomedicine and Biotechnology 2001;1(3):122-128.

Published online Jan 2001


Copyright © 2001, Hindawi Publishing Corporation

The gradient fractions were immunoprecipitated with anti-pORF2 antibodies and analyzed on nonreducing 3.5% polyacrylamide gels.