026.fig.002.jpg (18.72 kB)
Download fileCOS-1 cells transfected with wild type or Î2â111 ORF2 expression plasmids were lysed 48 hours post-transfection, the lysates cross-linked with glutaraldehyde and separated on a sucrose gradient as described in materials and methods
figure
posted on 2011-12-30, 22:12 authored by Li Xiaofang, Mohammad Zafrullah, Faizan Ahmad, Shahid JameelCopyright information:
Taken from "A C-terminal hydrophobic region is required for homo-oligomerization of the hepatitis E virus capsid (ORF2) protein"
Journal of Biomedicine and Biotechnology 2001;1(3):122-128.
Published online Jan 2001
PMCID:PMC129057.
Copyright © 2001, Hindawi Publishing Corporation
The gradient fractions were immunoprecipitated with anti-pORF2 antibodies and analyzed on nonreducing 3.5% polyacrylamide gels.
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