CD4⁺ T cell populations in cancer septic animals exhibit increased coinhibitory receptor expression relative to previously healthy septic controls.

CD4⁺ T cells isolated from splenocytes of previously healthy or cancer septic animals were analyzed for coinhibitory receptor expression by flow cytometry. A, Representative flow plots of LAG-3, BTLA, 2B4, and PD-1 expression. Frequencies (B) and absolute numbers (C) of LAG-3⁺, BTLA⁺, 2B4⁺, and PD-1⁺ CD4⁺ T cells from previously healthy vs. cancer septic animals. D, Representative flow plots of CD127 expression. MFI of CD127 expression on CD4⁺ T cells from previously healthy vs. cancer septic animals. n = 11/group. ***p<0.001, *p<0.05.