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CD4 T cells from MOG-primed WT animals were expanded in vitro in the presence of MOG peptide and IL-23 and adoptively transferred into WT and TL1A KO mice

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posted on 31.12.2011 by Bhanu P. Pappu, Anna Borodovsky, Timothy S. Zheng, Xuexian Yang, Ping Wu, Xingwen Dong, Shawn Weng, Beth Browning, Martin L. Scott, Li Ma, Lihe Su, Qiang Tian, Pascal Schneider, Richard A. Flavell, Chen Dong, Linda C. Burkly
(A) EAE clinical course in WT and TL1A KO mice ( = 11–12 mice per group). Results shown are compiled from two independent experiments. Disease severity was significantly reduced in KO mice (WT vs. KO mean cumulative score, P = 0.04; and mean maximal score, P = 0.05, as determined by the Mann Whitney nonparametric test), with no difference in disease incidence (WT, 9 out of 11 mice; KO, 10 out of 12 mice). Disease onset tended to be delayed (P = 0.06, as determined by the Student's two-tailed test). (B) Mononuclear infiltrating cells from the CNS of WT and TL1A KO mice (pools of five animals) at day 24 after adoptive transfer were restimulated with PMA plus ionomycin and analyzed by intracellular cytokine staining. The absolute number of IL-17– and/or IFN-γ–expressing CD4 T cells are shown.

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Taken from "TL1A–DR3 interaction regulates Th17 cell function and Th17-mediated autoimmune disease"

The Journal of Experimental Medicine 2008;205(5):1049-1062.

Published online 12 May 2008