CD44 expression mediates RSV infection.
HEp-2 or HEK-293 cells were infected with 0.5 MOI of RSV-KL19F (red). After 24 hours, the cells were stained with FITC mouse anti-human CD44 antibody (green) and gated for RSV expression (red). (Top panel) Flow cytometry analysis of HEp-2 cells infected with RSV-KL19F. (Bottom panel) Flow cytometry analysis of HEK-293 cells infected with RSV-KL19F. Result of a representative experiment is shown. Experiments were performed in triplicate.