Broad characterization of lymphoid and non-lymphoid tissues in NOD and NOD CNS1-/- mice.
(A) Quantification of Treg percentages in various tissues (pLN = pancreatic lymph node, mLN = mesenteric lymph node, LI LP = large intestine lamina propria). (B) Representative plot of CD4+CD8-Foxp3+ thymocytes showing Neuropilin-1 (Nrp1) and Helios expression. (C) Quantification of Nrp1+Helios+CD4+CD8-Foxp3+ Treg thymocytes. (D) Representative plot of CD4+Foxp3+ T cells expressing Nrp1 and Helios in the mesenteric lymph node (mLN). Quantification of Nrp1-Helios- (E), Nrp1- (F), and Helios- (G) Tregs in mLN and pancreatic lymph node (pLN), reflecting potential pTreg populations. Representative plot of CD4+Foxp3+ T cells expressing RORγt and Helios in the large intestine lamina propria (LI LP) of NOD (H) and NOD CNS1-/- (I) mice. Quantification of Helios- (J) and RORγt+ (K) Tregs in the LI LP, reflecting potential pTreg populations. CD25 was also stained, but it was not used to selectively exclude any CD4+Foxp3+ T cells from analysis. All figures show NOD and NOD CNS1-/- female mice, 8–13 weeks (n = 11).