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Brassinosteroids (BRs) promote the resistance to rice stripe virus disease (RSV) in rice.

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posted on 31.08.2020, 17:38 by Jinlong Hu, Jie Huang, Haosen Xu, Yongsheng Wang, Chen Li, Peizheng Wen, Xiaoman You, Xiao Zhang, Gen Pan, Qi Li, Hongliang Zhang, Jun He, Hongming Wu, Ling Jiang, Haiyang Wang, Yuqiang Liu, Jianmin Wan

(A) Representative images of endogenous BR increased mutant slender grain Dominant (slg-D) and its wild-type Dongjin (DJ) plants infested with RSV-carrying SBPH. Bar = 5 cm (top). Bar = 3 mm (bottom). (B) RSV incidence of slg-D and DJ at 30-day post infection (dpi) with RSV-carrying SBPH. (C) Detection of RSV CP RNA expression levels (upper panel) by quantitative RT–PCR assay and western blot analysis of the RSV coat protein (lower panel) in the RSV-infected plants of slg-D and DJ, respectively. OsHSP was used as loading control. (D) Representative images of rice seedling infested with RSV-carrying SBPH after pretreatment with 1 μM epibrassinolide (BL) or MOCK (0.1% Triton X-100). Bar = 5 cm (top). Bar = 3 mm (bottom). (E) RSV incidence of high susceptible rice cultivar Asominori after pretreatment with 1 μM BL or MOCK at 30 dpi with RSV-carrying SBPH. (F) Detection of RSV CP RNA expression levels (upper panel) by quantitative RT–PCR assay and western blot analysis of the RSV coat protein (lower panel) in RSV-infected rice seedling after pretreatment with 1 μM BL or MOCK, respectively. OsHSP was used as loading control. Data are shown as mean ± SEM (n = 3). **P < 0.01 by the Student’s t-test in B, C, E and F.

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