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Download fileBovine and human milk-derived exosome isolation via ExoQuick (EQ) precipitation and differential ultracentrifugation (DUC) methods.
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posted on 2021-09-30, 17:46 authored by Sanoji Wijenayake, Shafinaz Eisha, Zoya Tawhidi, Michael A. Pitino, Michael A. Steele, Alison S. Fleming, Patrick O. McGowanBovine and human milk-derived exosome isolation via ExoQuick (EQ) precipitation and differential ultracentrifugation (DUC) methods.
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tem sample preparationheterogeneous biological fluidcalnexin protein markersfour extraction protocolsxlink "> milkdetermine whether prelowest rna yieldhuman mdes isolatedbovine mdes isolatedwhole milk priortrizol ls methodunpasteurized bovine milkrna extractionunpasteurized bovinehuman milkhuman mdestrizol lsoptimized methodmde yieldbovine milkmilk prerna extractionsrna cleanwestern immunoblottingthree preserial filtrationresults indicatenta ),mde fractionationhighly complexexosome isolationdifferential ultracentrifugationderived exosomescontains nonchallenging duecellular debriscasein proteinsbetter suitedalso present
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