Both L- and D-enantiomers of PLNC8 αβ are effective against WNV_Kunjin.

The flavivirus Kunjin (KUNV, 1×10⁵), suspended in DMEM, was exposed to A- L-enantiomer or B- D-enantiomer of PLNC8 α or PLNC8 β at a final concentration of 20 μM or PLNC8 αβ (molar ratio 1:1) at a final concentration of 0.1, 1, 5, 10 and 20 μM for 1 h at 37°C. The suspension was then added to Vero cells and incubated for 1 h, followed by quantification of the viral load by performing crystal violet-based plaque assay. The indicated concentrations are in μM. Representative images of the virus plaques are presented above the bars. The virus particles are rapidly permeabilized by both enantiomers of PLNC8 αβ that decreased the viral load by >99.9%. The data (n = 3) are presented as mean with SEM and analysed using one-way ANOVA with Dunnett´s multiple comparison test (***p<0.001).