Body Weight and Experimental Design of Weight Perturbation Studies.
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Weight curves are presented for LF (solid circles; N = 18), HF (white squares; N = 16), CR (grey solid triangles; N = 20), and HF-LF (white diamonds; N = 25) mice (mean +/- SEM). Timing of physiological analyses is indicated by a black rectangle (48–51 weeks). Significant difference from LF is indicated by barred solid (HF), dashed (CR), and dotted lines (HF-LF); body weight of both CR and HF-LF groups were significantly different (P<0.0001) from HF mice at all time-points beside the initial time point for each mouse group. *** P<0.001.