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Biodistribution of plasmids in the lungs by intratracheal delivery.

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posted on 2017-04-05, 18:05 authored by Daniel Tsung-Ning Huang, Chun-Yi Lu, Pei-Lan Shao, Luan-Yin Chang, Jin-Yuan Wang, Yi-Hsuan Chang, Mei-Ju Lai, Ya-Hui Chi, Li-Min Huang

The intratracheal model was performed by administering the plasmids through a 2.5-mm human otoscope specula that was placed directly into the trachea via the mouth. Forty-eight hours after plasmid delivery, GFP proteins were observed in lung sections by immunohistochemistry (IHC) staining (black arrows). Negative controls at (a) 200X and (b) 400X with IHC staining and (c) 200X and (d) 400X.

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