Binding proteins of rTsSPc and Caco-2 cells by GST-pull down.

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posted on 2024-01-08, 18:29 authored by Yan Yan Song, Xin Zhuo Zhang, Bo Ning Wang, Yong Kang Cheng, Xin Guo, Xi Zhang, Shao Rong Long, Ruo Dan Liu, Zhong Quan Wang, Jing Cui

rTsSPc was co-incubated with soluble Caco-2 cell proteins to capture their interacting proteins. M: Low molecular weight protein marker. Each experiment was performed in triplicate. The arrow points to two protein bands with 36 and 55 kDa from the Caco-2 cell proteins captured and interacted with rTsSPc.

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western blot showed previous studies showed intestinal infective larvae infected murine intestine degraded tight junctions intestinal epithelial cell first natural barrier intestinal epithelial barrier rack1 inhibitor ho adherent protein e promoted larval invasion impeded larval invasion trichinella spiralis mediated larval invasion regulated rack1 expression iil surface proteins rtsspc activating erk1 larval invasion barrier function larval penetration spiralis consequently mediated activating mapk epithelial integrity vaccine target serine proteinase rtsspc bound regulating expression proteases secreted paracellular permeability molecular mechanisms mass spectrometry ip indicated interacted specifically interacted proteins gut epithelium gst pull expression levels es proteins directly hydrolyze completely elucidated also inhibited activated mapk 3 dpi 2 pathway 2 monolayer 2 cells

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Binding proteins of rTsSPc and Caco-2 cells by GST-pull down.

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