Autoreactive Treg and Tconv percentages between NOD and NOD CNS1^-/- mice.

Representative tetramer staining plots of CD4⁺Foxp3^- Tconvs (A) and CD4⁺Foxp3⁺ Tregs (B) for insulin peptide InsB 9:23 and hybrid insulin peptide (HIP) fusion of insulin and chromogranin A show differential tetramer binding between Tregs and Tconvs. Tetramer staining within pancreatic islets for InsB:9–23 p8E (n = 7) (C) and InsB:9–20 p8G (n = 10) (D) variants and Ins:ChgA HIP2.5 (n = 9) (E) and Ins:IAPP2 HIP6.9 (n = 7) (F) is quantified and compared between NOD and NOD CNS1^-/- pre-diabetic females aged 8–13 weeks (* = p < .05; ** = p < .005).