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Automated parallel isolation of all species of tRNA and four sRNAs

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posted on 2011-12-30, 17:08 authored by Kenjyo Miyauchi, Tomoya Ohara, Tsutomu Suzuki

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Taken from "Automated parallel isolation of multiple species of non-coding RNAs by the reciprocal circulating chromatography method"

Nucleic Acids Research 2007;35(4):e24-e24.

Published online 23 Jan 2007


© 2007 The Author(s).

Polyacrylamide gel electrophoresis of tRNAs, 4.5S and 6S RNAs isolated by the RCC instrument. RNAs were visualized by ethidium bromide staining. Several tRNAs migrated as doublet bands, which were conformers of the same tRNAs. Species of tRNAs are shown as single letter abbreviations for the corresponding amino acid and a number extension in the case of multiple tRNAs for the same amino acid. total RNA (asterisk) was used as a marker. Northern blot analysis of DsrA and SraH sRNA. Strong signals were observed in the eluted fraction (elution), weak signals were visible in the starting sample (load), and no signals were found in the flow through fraction (FT). Ethidium bromide (EtBr) staining is also shown. Most of the target RNAs were highly concentrated in the eluted fractions.